Biotechnology Eligibility Test (DBT BET) 2025 was conducted on 13 May 2025 by the Department of Biotechnology (DBT). This national level examination acts as an entrance for candidates seeking Junior Research Fellowship (JRF) and enters PhD. Program in biotechnology across India. The exam was conducted in computer-based tests (CBT) mode only in English, with a period of only 3 hours (10:00 am to 1:00 pm). The paper consisted of 200 multiple choice questions (MCQ), which were divided into two sections, with a total of 300 marks. Below is a wide DBT BET exam paper analysis 2025, including section-wise and subject-wise breakdown.
DBT JRF exam paper analysis 2025
The DBT BET 2025 examination was conducted smoothly in various test centers. The level of difficulty was reported to be moderate, with section A slightly easier than Section B. Most of the questions were concept-based, which required a strong understanding of fundamental biotechnology subjects. DBT JRF exam 2025, was conducted by the Department of Biotechnology (DBT) on 13 May 2025, a computer-based test (CBT), in which 200 MCQs were divided into Section A (50 mandatory questions on General Science, Chemistry, Mathematics, and Basic Biotechnology) and Section B (150 Questions, Advandand Topics, Moleclands, Moleclands, Moleclands, Molecland Topics, rullogind, molecland, rullocly, molecland, rullogind focusing 300 marks with a moderate difficulty level, where section A was relatively easy (NCERT-based), while section B demanded deeper knowledge.
DBT Condition Exam 2025 Student Response
The DBT BET exam 2024 was successfully conducted, and many candidates have shared their insight about paper levels, subject-wise distribution and type of questions asked. Based on the student response, the exam was easy to moderate difficulty. Below is a wide version of the exact reaction shared by the students:
- A question on Aspergillus Niger was asked in the ‘Match the Follow the’ format.
- Very few questions were asked about immunology.
- In enzymes, a question was asked on competitive prohibition. In this, Vmax was growing and KM was being asked.
- There were many questions from biochemistry – about 20 to 30 questions.
- Many ‘matches the following’ types of questions were included in the paper.
- The overall difficulty level of the exam was easier to moderate.
- A large number of questions were asked about microbiology.
Bet Exam Analysis – Question Review by students
The following questions were reported directly by students who appeared for the DBT BET exam 2025. These reflect the types of ideological and applicable subjects that appeared in the examination, especially from classes such as molecular biology, bio chemicals, techniques, and general science awareness. The phrase of these questions shows how the candidates remembered him after the exam. This analysis helps candidates understand the nature of questions and focus focus for future preparation.
- What is the full form of the explosion?
- Where is lipid synthesis – in a goal tool or endoplasmic reticulum?
- What is the purpose of dideoxinucleotides in Sanger sequencing?
- In Sengar sequencing, do we need to reverse the sequence to get the end result?
- Amino acid sequences were provided, and we were asked to identify which the highest absorption would show.
- Is Paneer a fermented product or a narrow product?
- Which amino acids are the precursor to vitamin niacin?
- Protein A was a trimmer and protein B was a monomer, both with a subjuity molecular weight of 20 KDA. We were asked to determine which protein would be first and which would end it later.
- Protein-DNA interaction is not studied in which of the following?
- What was the first transgenic crop?
- A question related to PKA and PH.
- How does athidium link to bromide DNA?
- In which vessel does bidary solutions transport take place?
- Calendar-based questions (3 in total 3).
- Where is white matter located?
- What is the first committed enzyme in glycolisis?
- Match the following: Curd, cheese, etc. (bioprocessing questions)
- Which is the weakest acid: acetic acid, HF, HNO, or HCN?
- Questions on age and relationships (2–3 questions).
- In agrobacterium tumpence, which two genes activate all others?
- What is the length of an alpha helix with 120 amino acids?
- Does histones have more arginine and lysine?
- Which amino acids cause fluorescence in proteins? (2 questions)
- Which amino acid contains sulfur?
- Questions on FTIR, UV and CD spectroscopy (2 questions).
- A question on NMR spectroscopy.
- Match the following: Chromatography theory (2 questions).
- Especially two questions on gel filtration chromatography.
- If the pH is less than PK, what is the nature of ions?
- Given the sedimentation coefficient, what is the relationship in terms of molecular load?
- What happens to DNA when grown in N15 for generations and then transferred to a generation in reference to the number of bands to N14?
- What do Aspergillus Niger, Lactobacillus, acetobacter and Sacrromes produce?
- Which of the following is not a zoonotic disease: cholera, plague, etc.?
- Match the following pathogens with diseases: anthrax, syphilis, leprosy, which cough, etc.
- Which technique is used to estimate HCG in pregnancy test?
- Which monoclonal antibody is not used for the treatment of cancer?
- How is blood group typing done in laboratories?
DBT Biotechnology Exam Schedule
The Department of Biotechnology (DBT) conducted the Biotechnology Eligibility Test (BET) 2025 on 13 May 2025 in the Computer-based Test (CBT) mode. The exam was held in the same shift from 10:00 am to 1:00 pm, candidates were required to report early for mandatory verification. Below is a detailed schedule for DBT BET 2025:
| events | Date time | Description |
|---|---|---|
| test date | 13 May 2025 | JRF and Ph.D. National level testing for. Admission |
| Reporting time | 8:30 am – 9:30 am | Biometric verification and seating system |
| exam period | 10:00 pm – 1:00 pm | 3-hour CBT Window (200 MCQs) |
| Method | Online (CBT) | Hand held in designated test centers |
Biotechnology Eligibility Test Test Examination Pattern
The exam pattern for DBT BET 2025 was as follows:
| Section | Number of questions | Per question mark | total marks | Topics covered |
|---|---|---|---|---|
| One segment | 50 (compulsory) | 3 | 150 | General Science, Mathematics, Chemistry, General Qualification, Analytic and Quantitative Ability, General Biotechnology |
| Section B | 150 (Efforts of any 50) | 3 | 150 | Special subject in biotechnology (molecular biology, genetics, immunology, etc.) |
DBT Bet Exat Paper Analysis 2025-Section-War
The DBT BET 2025 exam shows two different classes designed to evaluate both basic knowledge and specialized expertise in biotechnology. Block is a test general qualification and basic science, while Khand B focused on advanced biotechnology concepts. Below is a wide breakdown of section-wise difficulty level, major subjects and questions distribution:
| Section | Total Questions | difficulty level | Major topics covered |
|---|---|---|---|
| Section A (mandatory) | 50 questions (150 marks) | Easy to medium |
|
| Section B (50 out of 150 attempts) | 150 questions (150 marks) | Medium to hard |
|
DBT Bet Exat Paper Analysis 2025-Topic-Wise
Section A: Subject War Weightage
| Subject | Number of questions | difficulty level |
|---|---|---|
| general Science | 10 | Easy |
| chemistry | 8 | medium |
| Mathematics | 10 | medium |
| General biotechnology | 12 | Easy medium |
| Analytical and quantitative capacity | 10 | medium |
Section b subject-wise weightage
| Subject | difficulty level |
|---|---|
|
Organism
molecular Biology
Germination
plant Physiology
Immunology and disease
Biotechnology technology
food Technology Genetics Analytical technique |
Easy – medium |
Download DBBT BET Examper Paper Analysis 2025 PDF
Detailed questions, exam patterns, subjects are given below for DBT BET 2025 exam. Candidates can lower DBT BET exam paper analysis 2025 PDF through the link given below.
Download DBBT BET Examper Paper Analysis 2025 PDF
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